transcription

CRISPR Cas9 Synthetic Eukaryotic Transcription Factors

CRISPR Cas9 Synthetic Eukaryotic Transcription Factors

The CRISPR Cas9 technology is used to design synthetic transcription factors in eukaryotes. The approach is used to design activators and repressors. Data shows that this approach can work in yeast and mammalian cells. This paper comes fromĀ Tim Lu’s groupĀ at...

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

This looks like a great method to investigate the dynamics of molecular noise. Curious to compare it with FISH data.

Controlling Gene Expression Noise by Preventing Interchromosomal Interactions

Controlling Gene Expression Noise by Preventing Interchromosomal Interactions

In this paper, the authors examine intrinsic and extrinsic noise in the yeast pheromone response MAP kinase pathway. The transcription factor Ste12 is regulated by this kinase pathway, and there are two partially redundant repressors, Dig1 and Dig2, that interact...

Method of Gene Regulation Influences Intrinsic Noise Independently of Expression Levels

Method of Gene Regulation Influences Intrinsic Noise Independently of Expression Levels

Cell-to-cell variability in gene expression (a.k.a. gene expression noise) in a genetically identical population of cells is the result of a combination of extrinsic and intrinsic “noise”. Extrinsic noise is the result of variations in the cellular environment (e.g. cells...

Control of Stochasticity in Eukaryotic Gene Expression

Control of Stochasticity in Eukaryotic Gene Expression

This paper uses the dual reporter approach to measuring gene expression developed by Elowitz (see this journal club entry). The authors look at different promoters. In all cases, the intrinsic noise was a small fraction of the total noise. The...