single cell

Parameter estimation preprint

We just submitted a revised version of a manuscript describing the analysis of data collected using our GenoSIGHT software. The paper shows that this data can be used to estimate parameters of gene network models. This manuscript is under consideration...

Adaptive Imaging Cytometry

Adaptive Imaging Cytometry

The use of microfluidics in live cell imaging allows the acquisition of dense time-series from individual cells that can be perturbed through computer-controlled changes of growth medium. Systems and synthetic biologists frequently perform gene expression studies that require changes in...

Publishing preprint on adaptive imaging cytometry using Figshare

Today we submitted to PLoS ONE a manuscript entitled “Adaptive imaging cytometry to estimate parameters of gene networks models in systems and synthetic biology“. The journal has a new data sharing policy that encourages authors to deposit data in a...

Adaptive imaging cytometry to estimate parameters of gene networks models in systems and synthetic biology

Adaptive imaging cytometry to estimate parameters of gene networks models in systems and synthetic biology

Supplementary material to a manuscript under review. The manuscript can be downloaded from the link below. 

Abstract

Recent decades have yielded an understanding of cellular behavior as arising from a complicated soup of noisy molecular interactions. Systems biologists [...]

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Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

This looks like a great method to investigate the dynamics of molecular noise. Curious to compare it with FISH data.

Method of Gene Regulation Influences Intrinsic Noise Independently of Expression Levels

Method of Gene Regulation Influences Intrinsic Noise Independently of Expression Levels

Cell-to-cell variability in gene expression (a.k.a. gene expression noise) in a genetically identical population of cells is the result of a combination of extrinsic and intrinsic “noise”. Extrinsic noise is the result of variations in the cellular environment (e.g. cells...