DNA extraction protocol for yeast genomes or plasmids

Peccoud Lab Protocol: Isolating genomic DNA or plasmids from yeast Introduction The following protocol will recover total DNA and, with the indicated modifications, is useful to recover plasmids or genomic DNA. For plasmid recovery, the total DNA is transformed into E....

Coomassie blue staining protocol for protein gels

Peccoud lab Protocol: Staining protein gels with Bio-Safe Coomassie blue Introduction In gel visualization of proteins allows one to determine how evenly loaded the protein sample were, and the general state of the samples – i.e., are the bands sharp...

SDS-PAGE protocol: visualizing proteins on acrylamide gels

Peccoud Lab Protocol: visualizing proteins via sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) Introduction Because cellular extracts contain thousands of different proteins at a wide range of concentrations, it is often difficult to detect and measure specific proteins in these mixes, even when...

agarose gel electrophoresis protocol: visualizing DNA

Peccoud lab Protocol: Visualizing DNA following size exclusion on an agarose gel Introduction In agarose gel electrophoresis, a sample of a biological molecule, in this case, DNA, is loaded into a well formed in an agarose gel matrix. To prevent...

synthetic biology training

FBI Synthetic Biology Training

FBI Training Objectives For one week in May, we hosted 11 agents and analysts from the U.S. Federal Bureau of Investigation for an intensive synthetic biology training program. The goal of this event was to give the law enforcement personnel foundational...

Synthetic Biology Summer Schools

For graduate students interested in getting intensive training in synthetic biology, there are several summer schools worth considering: CSHL Summer School in Synthetic Biology: This program spans 2 weeks and combines lectures and intensive lab experience. Application deadline is April...

security of biomanufacturing

Security of biomanufacturing infrastructures

I am glad to be part of a multi-institutional team newly commissioned to analyze the security of biomanufacturing infrastructures.  Facilities that manufacture biologic drugs like vaccines are a critical part of the nation’s biodefense infrastructure. Possible breaches of data systems controlling...

synthetic biology training

Practical Introduction to Synthetic Biology Security

Source of Support: FBI Total Amount Requested: $40,000 Period Covered: 08/15/16 to 09/30/17 Description: We created a training module to brief biosecurity agents on the latest developments in synthetic biology and trained 11 federal agents in a weeklong course. The training...

1 2 3 4 5 14