synthetic biology journal

New synthetic biology journal

Why a new synthetic biology journal? Most synthetic biology papers are not published in a synthetic biology journal. They are still published in non-specialized journals that span a broad spectrum of scientific specialties from bioinformatics to molecular biology and biotechnology. It...

Phenol-chloroform extraction & EtOH precipitation protocol

Peccoud Lab Protocol: purifying and/or concentrating DNA/RNA from various sources by phenol-chloroform extraction and ethanol precipitation. Introduction Sometimes it becomes necessary to concentrate your DNA/RNA or obtain purer DNA/RNA samples. If your DNA is resuspended in nucease-free water, you can...

Primers for gene deletion in yeast (design protocol)

Peccoud Lab Protocol: how to design primers for generating a de novo gene deletion in yeast Introduction Yeast strains carrying single deletions of non-essential genes are commercially available from GE Life Sciences (http://dharmacon.gelifesciences.com/cdnas-and-orfs/non-mammalian-cdnas-and-orfs/yeast/yeast-knockout-collection/). However, these are available only as deletions...

DNA extraction protocol for yeast genomes or plasmids

Peccoud Lab Protocol: Isolating genomic DNA or plasmids from yeast Introduction The following protocol will recover total DNA and, with the indicated modifications, is useful to recover plasmids or genomic DNA. For plasmid recovery, the total DNA is transformed into E....

Coomassie blue staining protocol for protein gels

Peccoud lab Protocol: Staining protein gels with Bio-Safe Coomassie blue Introduction In gel visualization of proteins allows one to determine how evenly loaded the protein sample were, and the general state of the samples – i.e., are the bands sharp...

SDS-PAGE protocol: visualizing proteins on acrylamide gels

Peccoud Lab Protocol: visualizing proteins via sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) Introduction Because cellular extracts contain thousands of different proteins at a wide range of concentrations, it is often difficult to detect and measure specific proteins in these mixes, even when...

agarose gel electrophoresis protocol: visualizing DNA

Peccoud lab Protocol: Visualizing DNA following size exclusion on an agarose gel Introduction In agarose gel electrophoresis, a sample of a biological molecule, in this case, DNA, is loaded into a well formed in an agarose gel matrix. To prevent...

synthetic biology training

FBI Synthetic Biology Training

FBI Training Objectives For one week in May, we hosted 11 agents and analysts from the U.S. Federal Bureau of Investigation for an intensive synthetic biology training program. The goal of this event was to give the law enforcement personnel foundational...

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