New Hires

As the group is completing its transition to Colorado State University, we have been building a new team. Since May, we have added five new hires to our team. Jenny Harding is a research coordinator. She joined us from the Department...

gene deletion & gene fusion protocol for yeast

Peccoud Lab Protocols: using PCR to delete a yeast gene or fuse a tag to a gene Introduction Yeast gene deletions or gene fusions (e.g. ORF with epitope tags or FP, promoter replacement, MS2 mRNA tagging) can be generated simply by...

C-terminal tag protocol: Adding a C-terminal tag to a gene

Peccoud Lab Protocol: designing primers to add a C-terminal tag to the (3′) end of a gene via PCR Introduction It is sometimes necessary to generate gene fusion strains with a C-terminal tag or promoter changes by intoducing a PCR amplicon...

N-terminal tag protocol: Adding an N-terminal tag to a gene

Peccoud Lab Protocol: designing primers to add an N-terminal tag to the 5′ end of a gene via PCR Introduction It is sometimes necessary to generate gene fusion strains with an N-terminal tag or promoter changes by introducing a PCR amplicon...

synthetic biology journal

New synthetic biology journal

Why a new synthetic biology journal? Most synthetic biology papers are not published in a synthetic biology journal. They are still published in non-specialized journals that span a broad spectrum of scientific specialties from bioinformatics to molecular biology and biotechnology. It...

Phenol-chloroform extraction & EtOH precipitation protocol

Peccoud Lab Protocol: purifying and/or concentrating DNA/RNA from various sources by phenol-chloroform extraction and ethanol precipitation. Introduction Sometimes it becomes necessary to concentrate your DNA/RNA or obtain purer DNA/RNA samples. If your DNA is resuspended in nucease-free water, you can...

Primers for gene deletion in yeast (design protocol)

Peccoud Lab Protocol: how to design primers for generating a de novo gene deletion in yeast Introduction Yeast strains carrying single deletions of non-essential genes are commercially available from GE Life Sciences (http://dharmacon.gelifesciences.com/cdnas-and-orfs/non-mammalian-cdnas-and-orfs/yeast/yeast-knockout-collection/). However, these are available only as deletions...

DNA extraction protocol for yeast genomes or plasmids

Peccoud Lab Protocol: Isolating genomic DNA or plasmids from yeast Introduction The following protocol will recover total DNA and, with the indicated modifications, is useful to recover plasmids or genomic DNA. For plasmid recovery, the total DNA is transformed into E....

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