virology

Synthetic generation of influenza vaccine viruses for rapid response to pandemics

Synthetic generation of influenza vaccine viruses for rapid response to pandemics

During the 2009 H1N1 influenza pandemic, vaccines for the virus became available in large quantities only after human infections peaked. To accelerate vaccine availability for future pandemics, we developed a synthetic approach that very rapidly generated vaccine viruses from sequence...

Recombinant Vesicular Stomatitis Virus Transduction of Dendritic Cells Enhances Their Ability to Prime Innate and Adaptive Antitumor Immunity

Recombinant Vesicular Stomatitis Virus Transduction of Dendritic Cells Enhances Their Ability to Prime Innate and Adaptive Antitumor Immunity

In this paper, Boudreau et al. examined the effectiveness of using a mutant vesicular stomatitis virus (VSV) as a transduction and maturation vector for dendritic cells (DC) as an ex vivo vaccine of sorts. Briefly, cells from the bone marrow of...

Kinetics of virus production from single cells.

Kinetics of virus production from single cells.

This paper presents an interesting method to measure the kinetics of VSV replication from individual cells. The main benefit is that it is low-tech. The main drawback is that it is fairly labor intensive.